EN PRENSA. High Performance Liquid Chromatography coupled Diode Array Detector (HPLC-DAD) method development and validation for the quantification of triclosan in toothpaste. EN PRENSA

Triclosan (TCS) is used in many health care products as a broad-spectrum antimicrobial and disinfectant agent; however, it currently considered an emerging pollutant. The present study aimed to develop analytical HPLC method coupled Diode Array Detector for the TCS determination toothpaste. Method development involved optimization of separation sample preparation. For chromatographic optimization, different RP columns aqueous mobile phases were addressed. optimized was achieved around 3 minutes using Symmetry C18 column at 30 °C, 0.6 mL/min flow-rate, 225 nm wavelength detection. toothpaste preparation response surface methodology Box-Behnken fractional factorial design chosen. treatment consisted leaching 0.2 g by sonication 7 2 mL MeOH. According International Conference on Harmonization (ICH, 1996/2005) European Commission Decision 200/657/EC guidelines, developed validated. found fast, economic, linear, sensitive, precise, robust allowed quantify healthcare products.